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The antibody is from purified rabbit serum by affinity purification by way of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene has been noticed in lots of tumors, which ends up in chromosome instability, and thus may perhaps add to tumorigenesis. This protein was located to associate with, and become linked to, the phosphorylation of NPAT protein (nuclear protein mapped on the ATM locus), which participates in cell-cycle regulated histone gene expression and performs a vital role in marketing cell-cycle progression in the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinct isoforms, have been explained. Two further splice variants were noted but in depth nucleotide sequence info is not really nevertheless accessible.
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Blocking peptides are peptides that bind specially towards the focus on antibody and block antibody binding. These peptide usually is made up of the epitope regarded from the antibody.
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This guidebook outlines additional possible optimization of mobile and immunoassay parameters to be sure the best possible success are...
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This tutorial outlines even further probable optimization of mobile and immunoassay parameters to be certain the very best success are...
Antibodies sure to the blocking peptide now not bind to your epitope over the focus on protein. By evaluating the staining through the blocked antibody compared to the antibody by itself, one can see which staining is particular.
Goal Data Cyclin E belongs on the very conserved cyclin loved ones, whose associates are characterised by a extraordinary periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDK kinases. Distinctive cyclins show distinct expression and degradation styles which lead for the temporal coordination of each mitotic celebration. Cyclin E varieties a complex with and capabilities to be a regulatory subunit of CDK2, whose activity is required for mobile cycle G1/S changeover. This protein accumulates for the G1-S stage boundary and is also degraded as cells development by means of S phase.
The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by equally antibodies...
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